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Junko Hori, Nancy C. Joyce, J. Wayne Streilein; Immune Privilege and Immunogenicity Reside among Different Layers of the Mouse Cornea. Invest. Ophthalmol. Vis. Sci. 2000;41(10):3032-3042.
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purpose. To determine the extent to which each layer of the mouse cornea
displays alloimmunogenicity or immune privilege.
methods. Intact corneas or individual or combined layers of corneas from normal
or cauterized eyes of BALB/c, C57BL/6, and CD95L-deficient
B6-gld mice were grafted beneath the kidney capsule of
normal BALB/c, B10.D2, BALB.B mice or of BALB/c mice presensitized to
donor antigens. Graft fate was assessed clinically and histologically
and acquisition of donor-specific delayed hypersensitivity (DH) was
assessed at selected intervals after grafting.
results. Full-thickness allogeneic corneas induced vigorous DH and were rejected
acutely. Similar results were obtained with allografts of corneal
epithelium alone (if supported by syngeneic viable stroma), allografts
of epithelium from cauterized corneas (containing Langerhans’ cells),
and stromal allografts deprived of endothelium. Grafts comprised of
stroma plus endothelium (without epithelium) were not rejected, nor did
they induce DH unless the graft had no CD95L expression. If
stroma–endothelium grafts had no CD95L expression, DH directed against
major histocompatibility complex (MHC), but not minor
histocompatibility, alloantigens was induced. Moreover, CD95L expressed
on stroma–endothelium grafts protected endothelial cells, but not
stromal cells, from rejection in presensitized recipients.
conclusions. When grafted to a heterotopic site, the alloimmunogenicity of the
normal cornea resides within its epithelial and stromal layers, whereas
immune privilege arises from the endothelium. In normal mice,
CD95L-expressing endothelium can inhibit the stroma from inducing
immunity directed at MHC alloantigens, but in presensitized mice the
endothelium can protect itself only from immune
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