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Shigeki Fujii, Michael F. T. Escaño, Kazuki Ishibashi, Motoko Fujii, Yoshibumi Sekiya, Misao Yamamoto, Kiyofumi Saijoh; Differential Expression of Neuroendocrine-Specific Protein in Form-Deprived Chick Eyes. Invest. Ophthalmol. Vis. Sci. 2000;41(6):1533-1541.
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purpose. To identify genes that are highly expressed in form-deprived
retina–retinal pigment epithelium–choroid tissues.
Neuroendocrine-specific proteins were found to be highly expressed.
methods. mRNAs enriched in retina–retinal pigment epithelium–choroid tissues
from 3-, 7-, and 14-day form-deprived chick eyes were isolated by
differential display technique with cDNA library screening.
Neuroendocrine-specific protein A and C were cloned in control and
form-deprived eyes. mRNA and protein levels, with respective regional
localizations, were examined by Northern blot, Western blot, and
immunohistochemical analyses, respectively.
results. The isolated clone included an insert with a sequence homologous to
both chick neuroendocrine-specific proteins A and C. The increases in
mRNA and protein levels were confirmed by Northern and Western blot
analyses, respectively. Immunohistochemical localization of
neuroendocrine-specific proteins A and C was detected in the layer of
photoreceptor inner segments, presumably in the cone cells. Northern
blot analysis using negative lenses showed that levels of
neuroendocrine-specific protein A and C mRNAs were not altered using
conclusions. The expression of both neuroendocrine-specific proteins A and C mRNAs
in cone photoreceptor cells was upregulated within 14 days of form
deprivation, but not in response to negative spectacle lenses. These
data suggest that the increase in induction of neuroendocrine-specific
proteins is not a secondary consequence of ocular elongation or myopic
refraction. Induction of neuroendocrine-specific proteins in
form-deprived eyes may be causally related to the development of myopia
or may be an unrelated effect of form
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