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Srish Sinha, Ashish Sharma, Neeraj Agarwal, Anand Swaroop, Teresa L. Yang–Feng; Expression Profile and Chromosomal Location of cDNA Clones, Identified from an Enriched Adult Human Retina Library. Invest. Ophthalmol. Vis. Sci. 2000;41(1):24-28.
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purpose. To delineate the profile of genes expressed in the adult human retina
and assign chromosomal location of cDNA clones.
methods. The end-sequence of random clones from an enriched human retinal cDNA
library was analyzed by NCBI database search. Expression profile was
established by northern blot analysis, database search, or both.
Selected cDNA clones were localized to human chromosomes by somatic
cell hybrid analysis, in situ hybridization to metaphase chromosomes,
or both. Chromosomal location was also obtained by searching the
results. One hundred and thirty-seven clones were isolated from the subtracted
retinal library. Fifty-one clones were identical with 35 known human
genes in GenBank, and 24 clones corresponded to 23 uncharacterized
human expressed sequenced tags (ESTs), novel genes, or both. The
remaining 59 clones were not pursued further because they contained
bacterial sequences or repetitive elements. Several clones indicated a
restricted pattern of expression with high levels of transcripts in the
retina. Chromosomal location of novel retinal ESTs is also reported.
conclusions. This study provides a profile of genes expressed in the adult human
retina. One round of subtraction eliminated most constitutively
expressed genes and permitted partial normalization of the retinal
library. Twenty-three novel genes were identified. The combined
information obtained from expression analysis and chromosomal
localization of retinal cDNAs should be valuable in identifying
candidate genes for diseases involving retinal
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