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Morris L. J. Crawford, Ronald S. Harwerth, Earl L. Smith, Stephen Mills, Bryan Ewing; Experimental Glaucoma in Primates: Changes in Cytochrome Oxidase Blobs in V1 Cortex. Invest. Ophthalmol. Vis. Sci. 2001;42(2):358-364.
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purpose. To evaluate the effects of ganglion cell depletion from experimental
glaucoma on the relative metabolic activities of neurons in the
cytochrome oxidase blobs of V1 cortex in the macaque visual system.
methods. Monocular experimental glaucoma was induced in adult monkeys
(Macaca mulatta and Macaca fascicularis) by laser
application to the trabecular meshwork, increasing the intraocular
pressure. After other experiments, the primary visual cortices were
analyzed for functional excitation from surviving ganglion cells, as
indicated by cytochrome oxidase histochemistry.
results. Cytochrome oxidase reactivity was uniformly reduced in blobs with input
from the glaucomatous eye in a manner consistent with loss of known
afferent inputs. The average size of glaucomatous blobs in layers 2 and
3 of V1 cortex was reduced by half.
conclusions. Experimental glaucoma in monkeys reduces retinal input to the central
nervous system, thereby reducing the metabolic drive to downstream
targets, as indicated by the reduction in the size of cytochrome
oxidase blobs in layers 2 and 3 of V1 cortex. The pattern of cytochrome
oxidase loss within the blob was uniform, suggesting that all sources
of afferent input to the blobs were affected by experimental
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