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Ivana Kim, Anne M. Ryan, Richard Rohan, Shiro Amano, Stephanie Agular, Joan W. Miller, Anthony P. Adamis; Constitutive Expression of VEGF, VEGFR-1, and VEGFR-2 in Normal Eyes. Invest. Ophthalmol. Vis. Sci. 1999;40(9):2115-2121.
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purpose. The expression of vascular endothelial growth factor (VEGF) and its
high-affinity receptors VEGFR-1 and VEGFR-2 was investigated in normal
methods. Monkey and rat eyes were surgically removed in animals under deep
anesthesia and immediately prepared for study. Ocular VEGF, VEGFR-1,
and VEGFR-2 expression was studied using a combination of in situ
hybridization, northern blot analysis, immunohistochemistry,
immunoassay, and reverse transcription–polymerase chain reaction.
results. Steady state VEGF mRNA levels were detected in the normal vascularized
ocular tissues of the monkey: the conjunctiva, iris, retina, and the
choroid–retinal pigment epithelial complex. VEGF121 and
VEGF165 were the major isoforms identified. VEGF protein
was detected in the conjunctiva, retina, and the choroid–retinal
pigment epithelial complex. Retinal VEGF mRNA localized to the
ganglion, inner nuclear, and retinal pigment epithelial cell layers.
VEGF protein localized to these same layers and to the cones of monkey
retina. VEGFR-1 and VEGFR-2 mRNAs were detected in normal monkey iris,
retina, and the choroid–retinal pigment epithelial complex. In both
monkey and rat eyes, VEGFR-1 and VEGFR-2 mRNAs were localized to the
inner nuclear layer of the retina.
conclusions. VEGF, VEGFR-1, and VEGFR-2 are constitutively expressed in the
vascularized tissues of normal eyes.
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