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Zhong Dong, Malkhan Katar, Sarah Alousi, Richard S. Berk; Expression of Membrane-Type Matrix Metalloproteinases 4, 5, and 6 in Mouse Corneas Infected with P. aeruginosa . Invest. Ophthalmol. Vis. Sci. 2001;42(13):3223-3227.
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purpose. To investigate the expression and regulation of membrane-type matrix
metalloproteinases (MT-MMPs) 4, 5, and 6 in the mouse corneas infected
with Pseudomonas aeruginosa.
methods. C57BL/6J mice were intracorneally infected with P.
aeruginosa. The expression of MT4-, MT5-, and MT6-MMP was
detected at both the mRNA and protein levels by RT-PCR and immunoblot
analysis. Immunohistochemical staining was performed to localize the
expression of MT4- and MT5-MMP in the mouse corneas.
results. Expression of MT4- and MT5-MMP was detected in the normal (uninfected)
cornea by RT-PCR and immunoblot analysis. When infected with P.
aeruginosa, the corneas showed significant induction of each
MT-MMP. Localization of MT4- and MT5-MMP revealed that the expression
of MT5-MMP was restricted to the epithelial tissue in the normal
cornea, whereas the induced expression of MT4- and MT5-MMP was
predominantly in the substantia propria, which contained most of the
infiltrating cells. MT6-MMP expression was not detected in the
uninfected cornea but was upregulated in the infected corneas.
conclusions. Expression of MT4-, MT5-, and MT6-MMP was induced in corneas infected
with P. aeruginosa. Immunohistochemistry showed
predominant immunoreactivity of MT4- and MT5-MMP in the substantia
propria. Previous histologic studies have revealed different patterns
of inflammatory cell infiltration with an increased number of
polymorphonuclear neutrophils (PMNs) during the early stage of
inflammation and increased macrophages during the late stage. These
results indicate a good correlation between the overexpression of the
MT-MMPs in the infected corneas and the inflammatory response—that is,
leukocyte infiltration—indicating that inflammatory cells such as
macrophages and PMNs may play a role in the upregulation of MT-MMPs
during corneal infection, which in turn can cause the destruction of
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