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Daniel J. Salchow, Peter Gouras, Kentaro Doi, Stephen P. Goff, Eberhard Schwinger, Stephen H. Tsang; A Point Mutation (W70A) in the Rod PDEγ Gene Desensitizing and Delaying Murine Rod Photoreceptors. Invest. Ophthalmol. Vis. Sci. 1999;40(13):3262-3267.
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purpose. To examine the corneal electroretinogram (ERG) of transgenic mice (W70A
mice) carrying a point mutation (W70A) in the gene encoding for theγ
-subunit of rod cGMP phosphodiesterase (PDEγ).
methods. The ERG of W70A mice was compared with that of normal mice. Cone
responses were separated from rod responses by light adaptation,
whereas rod sensitivity was assessed by threshold stimulation with dim
light. Spectral sensitivity curves of the ERG were obtained using a
constant response criterion.
results. The ERG of the W70A mouse has a desensitized, delayed rod b-wave at
threshold, and a prolonged rod b-wave at higher flash intensities. The
a-wave is absent even at maximal stimulation. The cone ERG of the W70A
mouse is indistinguishable from that of normal mice. The spectral
sensitivity of the W70A mouse is maximal in the UV spectrum, in
contrast to the normal mouse, which is most sensitive in the green
region of the spectrum. This supports the interpretation of the results
as normal cone and abnormal rod function in the W70A mouse.
conclusions. The W70A mouse represents new model of stationary nyctalopia that can
be recognized by its unusual ERG features.
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