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Tomohiro Ikeda, Hidemi Ishii, Toshiyuki Higuchi, Keiko Sato, Yasuhito Hayashi, Kozo Ikeda, Yoshifumi Hirabayashi; Localization of Thrombomodulin in the Anterior Segment of the Human Eye. Invest. Ophthalmol. Vis. Sci. 2000;41(11):3383-3390.
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purpose. To localize thrombomodulin (TM) in the anterior segment of the human
eye. TM is a vascular endothelial cell surface glycoprotein that acts
as a cofactor for the thrombin-catalyzed activation of the
anticoagulant protease zymogen, protein C.
methods. Immunohistochemical methods were used to detect TM expression in
corneal epithelial cells, the lens epithelial cells, and other cells in
the anterior segment of the eye. The expression of TM was also examined
in cultured human corneal epithelial cells.
results. TM was expressed in corneal epithelial cells, corneal endothelial
cells, and nonpigmented ciliary epithelial cells, which are in direct
contact with the aqueous humor. TM was also expressed in cultured
corneal epithelial cells and showed cofactor activity. The amount of
the antigen in the cultured corneal cells was approximately one tenth
of that in human umbilical vein endothelial cells, but its specific
cofactor activity (75%) was comparable to that of TM in human
umbilical vein endothelial cells. The trabecular meshwork and
endothelial cells lining Schlemm’s canal also showed positive staining
conclusions. The TM in the cells that are in contact with the aqueous humor appears
to be involved in maintaining the fluidity of the aqueous humor. In
contrast, TM in cells that are not in contact with the aqueous humor
may function in regulating cell proliferation and/or
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