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Katia Del Rio–Tsonis, Stanislav I. Tomarev, Panagiotis A. Tsonis; Regulation of Prox 1 during Lens Regeneration. Invest. Ophthalmol. Vis. Sci. 1999;40(9):2039-2045.
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purpose. To determine the expression pattern of Prox 1 during the
process of lens regeneration in the urodele Notophthalmus
methods. Polymerase chain reaction was performed to amplify a partial newt Prox 1 sequence. In situ hybridization and immunodetection
methods were used to detect the Prox 1 mRNA and the Prox 1
results. Prox 1 mRNA was present in the retina and in the lens (in
the epithelium and bow region) of the intact eye. Prox 1 protein was
found to be predominantly present in the lens and dorsal iris of the
intact eye, although some trace levels of Prox 1 protein were detected
in the ventral iris as well. After lentectomy, expression of the mRNA
was also pronounced in the dorsal dedifferentiating iris and the
regenerating lens. The ventral iris also expressed Prox 1 but seemingly
at lower levels. Although Prox 1 protein showed upregulation in the
dorsal iris during the process of lens regeneration, trace levels were
also detected in the ventral iris. In the retina, Prox 1 protein was
distributed in horizontal cells of the inner nuclear layer, whereas the
mRNA was expressed in all layers of the retina.
conclusions. Prox 1 was unevenly distributed in the intact cells of the newt iris,
with significantly higher levels of Prox 1 protein present in the
dorsal versus the ventral margin. This protein was differentially
regulated during the process of lens regeneration, with obvious
upregulation in the dorsal iris. Prox 1 is the first transcriptional
factor to be shown to be regulated in the dorsal versus ventral iris
during the process of lens regeneration.
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