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Kozo Sasaki, Hidetoshi Ino, Tanemichi Chiba, Emiko Adachi-Usami; Light-Induced Apoptosis in the Neonatal Mouse Retina and Superior Colliculus. Invest. Ophthalmol. Vis. Sci. 1999;40(13):3079-3083.
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purpose. Apoptosis occurs naturally in the rodent retina and superior colliculus
(SC) during the neonatal period. The authors used mice to demonstrate
the dependency of this apoptosis on the light stimulation and the
methods. A number of apoptotic cells were counted in the retina and SC from a
group of newborn mice reared in constant darkness (DD group), a group
reared in normal light and dark conditions (LD group), and a group
reared in constant darkness up to P7 and then transferred to normal
condition (DD-to-LD group). Terminal deoxynucleotidyl
transferase-mediated biotin-dUTP nick end labeling (TUNEL) was used for
visualization of the apoptotic cells.
results. In the LD group, apoptotic cells significantly increased in the retinal
nuclear layers, including both the outer and inner nuclear layers, the
retinal ganglion cell layer, and SC at postnatal day 1 (P1) and
postnatal day 2 (P2). The number of apoptotic cells in the ganglion
cell layer and SC reached the maximum level at P1. In contrast, in the
DD group, an increase in the number of apoptotic cells was not
observed. At P9, no significant increase in the number of apoptotic
cells was observed in the outer nuclear layer, ganglion cell layer, and
SC either in the LD, DD, or DD-to-LD groups, but the LD and DD-to-LD
groups showed a significant increase in the inner nuclear layer
compared to the DD group.
conclusions. Apoptosis during the neonatal period in the mouse visual system is
induced by a light stimulus. This apoptosis was not induced after P7 in
the retinal ganglion cell layer and SC, even if excessive cells
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