Purchase this article with an account.
Anne Vaughan–Thomas, Sophie J. Gilbert, Victor C. Duance; Elevated Levels of Proteolytic Enzymes in the Aging Human Vitreous. Invest. Ophthalmol. Vis. Sci. 2000;41(11):3299-3304.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
purpose. To identify whether aging of human vitreous is accompanied by an
elevation in degradative enzymes within the tissue. methods. Human vitreous samples from donors aged 10 to 88 years were placed in
two groups based on donor age of less than or more than 50 years.
Homogenized samples were analyzed by gelatin substrate zymography for
matrix metalloproteinases (MMP). Serine proteinases were detected by
casein substrate zymography, and a specific antibody was used to
confirm the identity of, and to quantify, the serine proteinase, plasmin. results. Progelatinase A (ProMMP-2) was present in all the vitreous samples but
did not show an age-related increase. MMP-2 was also present at low
levels. Progelatinase B (ProMMP-9) was found in approximately 80% of
samples analyzed, but neither its presence nor level of activity was
age dependent. Of the serine proteinase activities detected, an enzyme
of approximately 80 kDa was identified by Western blot analysis as
plasmin(ogen). Quantitative analysis revealed a significant increase in
plasmin(ogen) with age. conclusions. This study shows there is an age-related increase in potential
degradative activity in human vitreous that may be responsible for
degenerative changes such as vitreous liquefaction. The data suggest
that increased levels of these enzymes precede, or are indicative of,
underlying ocular disease in some individuals.
This PDF is available to Subscribers Only