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Douglas Borchman, Frank J. Giblin, Victor R. Leverenz, Venkat N. Reddy, Li-Ren Lin, Marta Cecilia Yappert, Daxin Tang, Li Li; Impact of Aging and Hyperbaric Oxygen In Vivo on Guinea Pig Lens Lipids and Nuclear Light Scatter. Invest. Ophthalmol. Vis. Sci. 2000;41(10):3061-3073.
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purpose. To measure lipid compositional and structural changes in lenses as a
result of hyperbaric oxygen (HBO) treatment in vivo. HBO treatment in
vivo has been shown to produce increased lens nuclear light scattering.
methods. Guinea pigs, approximately 650 days old at death, were given 30 and 50
HBO treatments over 10- and 17-week periods, respectively, and the
lenses were sectioned into equatorial, cortical, and nuclear regions.
Lipid oxidation, composition, and structure were measured using
infrared spectroscopy. Phospholipid composition was measured using 31P-NMR spectroscopy. Data were compared with those
obtained from lenses of 29- and 644-day-old untreated guinea pigs.
results. The percentage of sphingolipid approximately doubled with increasing
age (29–544 days old). Concomitant with an increase in sphingolipid
was an increase in hydrocarbon chain saturation. The extent of normal
lens lipid hydrocarbon chain order increased with age from the
equatorial and cortical regions to the nucleus. These order data
support the hypothesis that the degree of lipid hydrocarbon order is
determined by the amount of lipid saturation, as regulated by the
content of saturated sphingolipid. Products of lipid oxidation
(including lipid hydroxyl, hydroperoxyl, and aldehydes) and lipid
disorder increased only in the nuclear region of lenses after 30 HBO
treatments, compared with control lenses. Enhanced oxidation correlated
with the observed loss of transparency in the central region. HBO
treatment in vivo appeared to accelerate age-related changes in lens
lipid oxidation, particularly in the nucleus, which possesses less
conclusions. Oxidation could account for the lipid compositional changes that are
observed to occur in the lens with age and cataract. Increased lipid
oxidation and hydrocarbon chain disorder correlate with increased lens
nuclear opacity in the in vivo HBO model.
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