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Michael J. Giese, David C. Shum, Sylvia A. Rayner, Bartly J. Mondino, Judith A. Berliner; Adhesion Molecule Expression in a Rat Model of Staphylococcus aureus Endophthalmitis. Invest. Ophthalmol. Vis. Sci. 2000;41(1):145-153.
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purpose. To determine whether Staphylococcus aureus and its
components induce expression of E-selectin and intercellular adhesion
molecule (ICAM)-1 in rat ocular tissues and on human endothelial cells
methods. Experimental and control rat eyes were injected with 80 colony-forming
units of viable S. aureus and lipopolysaccharide-free
sterile saline (NS), respectively. Eyes were enucleated and immediately
frozen. E-selectin and ICAM-1 expression were evaluated on frozen
sections by using standard immunohistochemical techniques. Using an
enzyme-linked immunoassay, in vitro expression of E-selectin and ICAM-1
was evaluated on macrovascular endothelial cells after stimulation with S. aureus and selected purified components.
results. In S. aureus–injected eyes, E-selectin and ICAM-1
expression peaked at six to 24 hours, decreased slightly at 24 and 48
hours, and further declined by 72 hours. However, in NS-injected eyes,
peak levels of E-selectin and ICAM-1 were seen at 6 hours, after which
expression declined in the areas in which an increase was previously
observed. In in vitro assays, peptidoglycan (0.01 μg/ml) induced a
fourfold increase in E-selectin (P < 0.0001) and a
twofold increase in ICAM-1 (P < 0.002) expression.
Ribitol teichoic acid (RTA) (1 μg/ml) induced a twofold increase in
E-selectin (P < 0.0001) and a threefold increase
in ICAM-1 (P < 0.0001) expression.
conclusions. Eyes injected with S. aureus demonstrated a more intense
and prolonged expression of both E-selectin and ICAM-1 than did eyes
injected with NS. In addition, S. aureus components
induced the in vitro expression of these adhesion molecules on
macrovascular endothelial cells. The relevance of these findings to
microvascular endothelial cells is yet to be
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