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Bruce E. Cohan, David F. Bohr; Goldmann Applanation Tonometry in the Conscious Rat. Invest. Ophthalmol. Vis. Sci. 2001;42(2):340-342.
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purpose. To determine whether the Goldmann applanation tonometer can be modified to
measure intraocular pressure (IOP) in the conscious rat.
methods. In anesthetized rats Goldmann tonometers were tested that had reduced
biprism angles in the applanating tips and reduced weights in the
tonometer body from those used in humans and species with similar size
eyes. Tonometers with tips with biprism angles of 48° and an applied
weight of 25 mg per Goldmann scale division (2 g full scale) were
calibrated for the rat against manometrically measured IOP. Tonometers,
thus modified, were then used in conscious, unsedated rats.
results. In conscious rats the measured mean Goldmann value was 15.5 ± 0.6
mm Hg (confidence interval = 14.1, 16.6 mm Hg). This was the
plateau level reached after the repeated applanations (approximately
10) required to eliminate an artifactual decline in initial Goldmann
readings, which was larger than that in humans.
conclusions. The Goldmann applanation tonometer was modified to measure IOP in the
conscious, unsedated rat. This instrument, the standard for measuring
this key physiological parameter in the human eye, can now be applied
to the laboratory rat. This may advance the use of this important
animal as a model in IOP and glaucoma research.
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