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Hartmut Wenkel, Peter W. Chen, Bruce R. Ksander, J. Wayne Streilein; Immune Privilege Is Extended, then Withdrawn, from Allogeneic Tumor Cell Grafts Placed in the Subretinal Space. Invest. Ophthalmol. Vis. Sci. 1999;40(13):3202-3208.
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purpose. To determine whether the subretinal space can extend immune privilege
to allogeneic tumor cell grafts that do not possess their own inherent
methods. P815 tumor cells were injected into the anterior chamber (AC), the
subretinal (SR) space, or subconjunctivally in eyes of BALB/c
(allogeneic), SCID (immune incompetent), normal DBA/2
(syngeneic), or DBA/2 mice presensitized with P815 cells transfected
with interleukin-12 and B7.1. Tumor growth was observed clinically and
histologically for up to 50 days. BALB/c recipients were tested for
suppression of DBA/2-specific delayed hypersensitivity and concomitant
immunity. The SR space of tumor-containing eyes was assessed for its
capacity to support ovalbumin (OVA)-specific anterior chamber
associated immune deviation (ACAID).
results. P815 cells injected into the SR space of presensitized and normal DBA/2
and SCID mice grew progressively, resulting eventually in recipient
death. Tumor cells injected into the SR space of eyes of BALB/c mice
grew progressively until day 14, followed by tumor regression resulting
in phthisis bulbi (14/35) or tumor elimination (19/35)
with preserved ocular anatomy by day 35. Despite elimination of tumors
from the SR space, BALB/c recipients exhibited DBA/2-specific ACAID and
concomitant immunity. In addition, OVA injected into the SR space of
eyes from which tumor has been eliminated induced ACAID.
conclusions. Various parameters of immune privilege, originally described for the
AC, are characteristic of immune privilege within the SR space.
However, because P815 cells placed in the AC prove lethal for BALB/c
recipients, but P815 cells placed in the SR space resolve without
jeopardizing the host’s life, immune privilege in the SR space can be
distinguished from immune privilege in the AC, and this may have
implications for grafts of retinal tissue placed within the SR
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