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Paul A. Mieyal, Michael W. Dunn, Michal Laniado Schwartzman; Detection of Endogenous 12-Hydroxyeicosatrienoic Acid in Human Tear Film. Invest. Ophthalmol. Vis. Sci. 2001;42(2):328-332.
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purpose. Increased production of 12-hydroxyeicosatetraenoic acid[
12(R)-HETE] and 12-hydroxyeicosatrienoic acid[
12(R)-HETrE] positively correlates with the in vivo
progression of ocular surface inflammation in rabbits. Tear film was
collected from human subjects with inflamed eyes to determine whether
these eicosanoids could be detected from endogenous sources.
methods. Control and inflamed eyes were assessed and assigned a subjective
inflammatory score. Tears were collected and extracted with an internal
standard. Single-ion–monitoring gas chromatography–mass spectrometry
(SIM-GC-MS) was performed to quantitate endogenous levels of 12-HETE
results. 12-HETrE was detected in the tear film of both control and inflamed
eyes, with the mean level being seven times higher in inflamed tears.
12-HETE was not detected in control tears and was detected in only 6 of
38 inflamed-eye tear samples.
conclusions. The current findings demonstrate that the human eye produces detectable
amounts of 12-HETrE, which is released into the tear flow. The
increased levels of 12-HETrE associated with ocular surface
inflammation suggest that this eicosanoid may contribute to
inflammation of the ocular surface in humans.
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