Purchase this article with an account.
Saaeha Rauz, Elizabeth A. Walker, Cedric H. L. Shackleton, Martin Hewison, Philip I. Murray, Paul M. Stewart; Expression and Putative Role of 11β-Hydroxysteroid Dehydrogenase Isozymes within the Human Eye. Invest. Ophthalmol. Vis. Sci. 2001;42(9):2037-2042.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
purpose. The human eye is an important target tissue for steroid hormones, and
glucocorticoids have been implicated in the pathogenesis of ocular
disease, including glaucoma. In peripheral tissues, corticosteroid
hormone action is regulated at a prereceptor level through the activity
of the 11β-hydroxysteroid dehydrogenase (11β-HSD) isozymes: an
oxo-reductase (11β-HSD1) that activates cortisol (F) from cortisone
(E) and a dehydrogenase (11β-HSD2) that inactivates F to E. The
purpose of this study was to analyze the expression and putative role
of 11β-HSD within the human eye.
methods. Immunohistochemical and reverse transcription–polymerase chain
reaction (RT-PCR) studies were performed on sections of human ocular
tissues, surgical trabecular meshwork (TM) specimens and a ciliary
nonpigmented epithelial (NPE) cell-line. Free F and E concentrations in
aqueous humor were determined by gas chromatography-mass spectrometry
(GC/MS). IOP was measured in eight male volunteers before and after
oral ingestion of carbenoxolone (CBX), a known inhibitor of 11β-HSD.
results. 11β-HSD1 was expressed in the basal cells of the corneal epithelium
and the NPE. 11β-HSD2 was restricted to the corneal endothelium.
RT-PCR revealed mRNA for only the glucocorticoid receptor (GR) in the
TM specimens, whereas GR, mineralocorticoid receptor and 11β-HSD1
mRNAs were all present in the NPE cell line. The demonstration of free
F in excess of E (F/E 14:1) in the aqueous humor suggested predominant
11β-HSD1 activity. Compared with baseline (14.7 ± 1.06 mm Hg,
mean ± SD), the IOP decreased significantly on both the third and
seventh days of CBX ingestion (12.48 ± 1.11 mm Hg, P < 0.0001 and 11.78 ± 1.50 mm Hg, P < 0.0001, respectively).
conclusions. These results suggest that the 11β-HSD1 isozyme may modulate
steroid-regulated sodium transport across the NPE, thereby influencing
This PDF is available to Subscribers Only