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Akiko Yokoyama, Toshiyuki Oshitari, Hisanari Negishi, Mari Dezawa, Atsushi Mizota, Emiko Adachi-Usami; Protection of Retinal Ganglion Cells from Ischemia-Reperfusion Injury by Electrically Applied Hsp27. Invest. Ophthalmol. Vis. Sci. 2001;42(13):3283-3286.
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purpose. To determine whether the Hsp27 protein can rescue retinal ganglion
cells (RGCs) of rats from ischemia–reperfusion injury.
methods. Retinal ischemia was induced in rats by clamping the ophthalmic artery
within the dural sheath of the optic nerve. Immediately after removing
the clamp and beginning the reperfusion, Hsp27 protein solution was
injected into the vitreous, and electroporation was applied. To
determine whether Hsp27 entered the RGCs, anti-Hsp27
immunohistochemistry was performed. The retinal damage was evaluated by
counting the number of RGCs retrogradely labeled by
(diI) injected into the superior colliculus, and also by comparing the
ratio of TUNEL-positive to all RGCs in the RGC layer.
results. Electroporation successfully delivered Hsp27 protein into RGCs. In the
Hsp27 electroinjected group, the number of RGCs 7 days after
ischemia–reperfusion was significantly higher than in the control
groups. The ratio of TUNEL-positive cells to all RGCs was lower in the
group electroinjected with Hsp27 protein.
conclusions. Electroporation of Hsp27 protein into RGCs increased the resistance of
the RGCs to the apoptosis induced by ischemia–reperfusion
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