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Paul A. Knepper, Chandra S. K. Mayanil, William Goossens, Robert D. Wertz, Cory Holgren, Robert Ritch, R. Rand Allingham; Aqueous Humor in Primary Open-Angle Glaucoma Contains an Increased Level of CD44S. Invest. Ophthalmol. Vis. Sci. 2002;43(1):133-139.
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purpose. To determine whether the cell adhesion molecule CD44, the principal
receptor of hyaluronan, is altered in the aqueous humor and the
anterior segment of patients with primary open-angle glaucoma (POAG).
methods. The trabecular meshwork (TM), iris, ciliary body, and sclera of POAG
and age-matched control eyes preserved in ethanol were microdissected
and subjected to 1% Triton X-100 solubilization at 4°C. Western blot
analysis was performed using monoclonal antibodies that recognize
either CD44H (hematopoietic; extracellular domain) or CD44S (soluble
ectodomain). The concentration of soluble CD44S in aqueous and
microdissected tissues was measured by enzyme-linked immunosorbent
results. ELISA of soluble CD44S of aqueous from eyes of patients with POAG
indicated that the concentration of soluble CD44S is increased in
comparison with that of aqueous from normal eyes (P < 0.0003). Western blot analysis and densitometry of POAG iris and
ciliary body revealed a statistically significant increase in the
Triton X-100 extraction of CD44H. The predominant increases were in the
180-kDa (P < 0.001) and the 85-kDa
(P < 0.001) forms. ELISA of soluble CD44S
indicated that the concentration is statistically decreased in iris
(P < 0.05), ciliary body (P <
0.001), and TM (P < 0.005) of POAG eyes.
conclusions. Increased amounts of soluble CD44S in POAG aqueous and Triton
X-100–solubilized CD44H characterized POAG in the iris and ciliary
body. These soluble CD44 isoforms may influence the activity of the
transmembrane CD44H by acting as inhibitors of CD44H and, thereby,
adversely influence the cell survival of TM and retinal ganglion cells
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