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Tomo Suzuki, Yoichiro Sano, Shigeru Kinoshita; Effects of 1α,25-Dihydroxyvitamin D3 on Langerhans Cell Migration and Corneal Neovascularization in Mice. Invest. Ophthalmol. Vis. Sci. 2000;41(1):154-158.
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purpose. To examine the effects of 1α,25-dihydroxyvitamin D3 (1α,25[OH]2D3), a hormone that has
immunosuppressive properties, on Langerhans cell (LC) migration and
corneal neovascularization in mouse corneas.
methods. Two 10-0 nylon interrupted sutures were placed in the center of 50
BALB/c mouse corneas to induce LC migration and corneal
neovascularization. The mice were then randomly assigned to one of five
groups. Three groups (n = 11, n = 11, n = 6) received topical
1α,25(OH)2D3 (at concentrations of
10−7 M, 10−8 M, 10−9 M), one
group (n = 11) received vehicle only, and one group
(n = 11) received no eye drops. Instillation (three
times a day) began on the first day after suturing. Corneal
neovascularization was assessed by slit lamp microscopy and scored
according to the length of newly formed corneal vessels. Fourteen days
after suturing, the number of LCs that had migrated into the central
corneal epithelium was counted by an immunofluorescence assay using an
results. The number of LCs in the central cornea was 21.9 ± 2.8
cells/mm2 in the nontreated group and 17.8 ± 3.9
cells/mm2 in the vehicle-only group. Significantly fewer
LCs were detected in all groups that had received
1α,25(OH)2D3 compared with the vehicle only
and nontreated groups (10−7 M: 7.4 ± 1.2
cells/mm2, 10−8 M: 7.2 ± 2.0
cells/mm2, 10−9 M: 6.2 ± 0.7
cells/mm2). Moderate inhibition of corneal vascularization
was observed in the 10−7 M
1α,25(OH)2D3 group, but not the other groups.
conclusions. Topical administration of 1α,25(OH)2D3 can be
effective in suppressing ocular surface inflammation by inhibiting LC
migration into mouse corneas.
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