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David M. Wu, Todd Schneiderman, Jason Burgett, Parag Gokhale, Linda Barthel, Pamela A. Raymond; Cones Regenerate from Retinal Stem Cells Sequestered in the Inner Nuclear Layer of Adult Goldfish Retina. Invest. Ophthalmol. Vis. Sci. 2001;42(9):2115-2124.
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purpose. To determine whether retinal progenitor cells in the inner nuclear
layer give rise to regenerated cones after laser ablation of
photoreceptors in adult goldfish retina.
methods. Using a technique developed previously in this laboratory,
photoreceptors in the retina of adult goldfish were ablated with an
argon laser. The mitotic marker, bromodeoxyuridine, was used to label
proliferating and regenerated cells, which were identified with
results. Cells proliferating locally within lesion included microglia,
Müller glia, and retinal progenitors in the inner nuclear layer
(INL). The nuclei of both Müller glia and associated retinal
progenitors migrated from the inner to the outer nuclear layer. The
proliferating retinal progenitors, which express Notch-3 and N-cadherin, regenerated cone photoreceptors and then
conclusions. Previous work has demonstrated that photoreceptors in the goldfish
retina regenerate selectively after laser ablation, but the source of
regenerated cones has not been identified. The results reported here
provide support for the existence of retinal stem cells within the
adult fish retina that are capable of regenerating cone photoreceptors.
The data also support the involvement of Müller glia in the
production of regenerated cones.
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