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Yuko Seko, Yoshinori Seko, Hajime Fujikura, Jijing Pang, Takashi Tokoro, Hitoyata Shimokawa; Induction of Vascular Endothelial Growth Factor After Application of Mechanical Stress to Retinal Pigment Epithelium of the Rat In Vitro. Invest. Ophthalmol. Vis. Sci. 1999;40(13):3287-3291.
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purpose. To investigate the response to mechanical stress of vascular
endothelial growth factor (VEGF) production by cultured retinal pigment
epithelial (RPE) cells.
methods. A pulsatile stretch device was used in vitro. RPE cells of the second
passage were seeded onto flexible-bottomed culture plates; then, at
subconfluent culture, the plates were subjected to pulsatile stretch.
Culture plates prepared in the same way but not subjected to stretch
were used as controls. After stretching for 1 hour or 24 hours,
conditioned medium for measurement of VEGF production by RPE
cells was collected using a mouse VEGF immunoassay. To study the
expression of VEGF in RPE cells, passaged-cultured RPE cells were
exposed to pulsatile stretch for 0, 1, 3, or 14 hours. Total
cytoplasmic RNA was then prepared from the RPE cells. Northern blot
analysis was performed for VEGF, with G3PDH used as an internal
results. The expression and secretion of VEGF in RPE cells were increased by
conclusions. Results indicate that stretching of the RPE could result in increased
production of VEGF, with associated risk for neovascularization and
changes in the blood-retinal barrier.
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