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Farid Ahmed, Mario Torrado, Elaine Johnson, John Morrison, Stanislav I. Tomarev; Changes in mRNA Levels of the Myoc/Tigr Gene in the Rat Eye after Experimental Elevation of Intraocular Pressure or Optic Nerve Transection. Invest. Ophthalmol. Vis. Sci. 2001;42(13):3165-3172.
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purpose. To isolate the rat Myoc/Tigr gene and
investigate changes in its expression pattern in normal eyes and in
eyes with either pressure-induced optic nerve damage or optic nerve
methods. Expression pattern of the rat Myoc/Tigr gene was investigated by Northern blot hybridization. Optic nerve
damage and death of ganglion cells in the retina were induced
unilaterally, by injection of hypertonic saline solution, episcleral
vein cauterization, or optic nerve transection. The levels of mRNA for Myoc/Tigr were compared between several
tissues of the control and surgically altered eyes, by using
semiquantitative RT-PCR, real-time PCR, and Northern blot analysis.
results. The rat Myoc/Tigr gene is 10 kb long and
contains three exons. Among the eye tissues analyzed, Myoc/Tigr mRNA was detected in the
combined tissues of the eye angle, sclera, cornea, retina, and optic
nerve head. With pressure-induced optic nerve degeneration, the level
of Myoc/Tigr mRNA decreased in the retina
and the combined tissues of the eye angle, but increased in the optic
nerve head. After optic nerve transection, the level of Myoc/Tigr mRNA increased in the retina,
but did not change in the combined tissues of the eye angle.
conclusions. The decreased level of Myoc/Tigr mRNA in
the retina after induction of elevated intraocular pressure compared
with that in the control retina cannot be explained by ganglion cell
death alone. Differences in Myoc/Tigr mRNA levels in eye tissues after elevation of intraocular pressure or
optic nerve transection may reflect the activation of different
signaling pathways involved in regulation of this
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