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Rosella Cristiani, Gigliola Fontanesi, Giovanni Casini, Cristina Petrucci, Cecile Viollet, Paola Bagnoli; Expression of Somatostatin Subtype 1 Receptor in the Rabbit Retina. Invest. Ophthalmol. Vis. Sci. 2000;41(10):3191-3199.
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purpose. To detect mRNAs for somatostatin (somatotropin release-inhibiting
factor [SRIF]) receptor subtypes 1 to 5 (sst1 through
sst5) in rabbit retinas by reverse
transcription–polymerase chain reaction (RT-PCR) and to investigate
the distribution of sst1 by single- and double-label
methods. Semiquantitative RT-PCR using sst-specific primers from mouse sequences
was performed. sst1 was localized using a polyclonal
antiserum directed to human sst1 in cryostat sections of
retinas from either normal or optic nerve–transected animals.
Immunolabeled cell sizes and densities were measured in wholemounted
retinas using computer-assisted image analysis. Double-label
immunofluorescence was performed using the sst1 antiserum
in conjunction with monoclonal antibodies directed to SRIF, tyrosine
hydroxylase (TH), parvalbumin (PV), or γ-aminobutyric acid (GABA).
results. With RT-PCR it was found that all five sst mRNAs were expressed in the
rabbit retina, with highest levels of sst1 mRNA.
sst1 immunolabeling was localized to amacrine cells in the
proximal inner nuclear layer (INL) of all retinal regions and to
displaced amacrine cells in the ganglion cell layer (GCL) of the
ventral retina. Some large sst1-immunoreactive (IR) somata
were also present in the GCL. They were not observed after optic nerve
transection. Double-label immunofluorescence showed sst1 expression by all TH-IR amacrine cells and by other amacrine cells that
were neither PV-IR nor GABA-IR. In addition, sst1 was
expressed by all SRIF-containing displaced amacrine cells.
conclusions. All five sst mRNAs are expressed in the rabbit retina. The localization
of sst1 suggests that it may mediate SRIF actions onto
amacrine (including dopaminergic) and sparse ganglion cells.
sst1 expression in SRIF-IR cells suggests that this
receptor may also act as an autoreceptor.
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