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Hui-Rong Jiang, Lynne Lumsden, John V. Forrester; Macrophages and Dendritic Cells in IRBP-Induced Experimental Autoimmune Uveoretinitis in B10RIII Mice. Invest. Ophthalmol. Vis. Sci. 1999;40(13):3177-3185.
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purpose. To investigate the characteristics of the mononuclear cell infiltrate
in murine experimental autoimmune uveoretinitis (EAU).
methods. EAU was induced by immunization with bovine interphotoreceptor retinal
binding protein (IRBP) in Freund’s complete adjuvant (subcutaneous
injection) and pertussis toxin (intraperitoneal injection) in B10RIII
mouse. Then animals were killed on days 7, 9, 12, 15, 20, 26, and 39
after immunization. Eyes were processed for hematoxylin and eosin
staining to characterize the disease and to assess the severity and
extent of the EAU. Single and dual immunohistochemical staining in
various combinations with monoclonal antibodies against CD45, CD4, CD8,
major histocompatibility complex (MHC) class II, CD11c, NLDC-145, and a
variety of macrophage markers was performed.
results. The authors’ results showed that vitritis, vasculitis and
perivasculitis, retinal detachment, and granuloma formation in retina
and choroid were the predominant features of IRBP-induced B10RIII mice
EAU. Immunohistologic results showed that CD4+ T cells and
macrophages were the main infiltrating cells in retina and choroid
throughout the entire course of the disease. MHC class II negative
macrophages expressing antigens reacting with MOMA-2, F4/80,
sialoadhesin, and CD11b were prominent during the peak phase of tissue
damage in the retina and choroid. Dendritic cells (DCs) characterized
by dual positivity for MHC class II and CD11c and negative for
sialoadhesin appeared at time of disease onset and continued to be
recruited during the inflammatory process. DCs at the site of
inflammation were NLDC-145 weak and CD8 negative, indicating that they
were of the myeloid rather than the lymphoid lineage.
conclusions. The results suggest that EAU in B10RIII mice is initiated by
local-infiltrating, dendritic antigen–presenting cells, whereas tissue
damage is associated with sialoadhesin-positive, phagocytic
nonantigen-presenting macrophages during the effector
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