Purchase this article with an account.
John Khadem, Amadeo A. Veloso, Felipe Tolentino, Tayyaba Hasan, Michael R. Hamblin; Photodynamic Tissue Adhesion with Chlorin e6 Protein Conjugates. Invest. Ophthalmol. Vis. Sci. 1999;40(13):3132-3137.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
purpose. To test the hypothesis that a photodynamic laser-activated tissue
solder would perform better in sealing scleral incisions when the
photosensitizer was covalently linked to the protein than when it was
methods. Conjugates and mixtures were prepared between the photosensitizer
chlorin e6 and various proteins (albumin,
fibrinogen, and gelatin) in different ratios and used to weld
penetrating scleral incisions made in human cadaveric eyes. A
blue-green (488–514 nm) argon laser activated the adhesive, and the
strength of the closure was measured by increasing the intraocular
pressure until the wound showed leakage.
results. Both covalent conjugates and noncovalent mixtures showed a light
dose-dependent increase in leaking pressure. A preparation of albumin
chlorin e6 conjugate with additional albumin
added (2.5 protein to chlorin e6 molar ratio)
showed significantly higher weld strength than other protein conjugates
conclusions. This is the first report of dye–protein conjugates as tissue solders.
These conjugates may have applications in
This PDF is available to Subscribers Only