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Donald Tiang-Hwee Tan, Yan-Ping Liu, Li Sun; Flow Cytometry Measurements of DNA Content in Primary and Recurrent Pterygia. Invest. Ophthalmol. Vis. Sci. 2000;41(7):1684-1686.
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purpose. To evaluate DNA content and cellular proliferation rates in primary and
methods. Matched pterygium and superior conjunctiva tissue were obtained in 36
eyes of 36 patients undergoing pterygium excision with conjunctival
autografting (24 primary pterygia, 12 recurrent pterygia). Epithelial
and fibrovascular layers were separated for analysis. Matched superior
conjunctiva obtained at the time of surgery were used as controls.
Samples were prepared according to Thompson’s method, and flow
cytometry was performed with a Becton–Dickinson FACScan. Analysis of
histograms and calculations of cell percentages in cell cycle phases
were carried out using CellFit software (version 2.0). Mean
proliferation indices (MPIs) were compared using the Wilcoxon
matched-pair signed-rank test.
results. The MPI of pterygium fibrovascular tissue (13.4) was significantly
higher than the MPI of pterygium epithelium (3.1; P =
0.0001). The MPI of pterygium fibrovascular tissue was also
significantly higher than that of superior conjunctival fibrovascular
tissue (6.0; P = 0.0001). There was no difference in
MPI values between pterygium epithelium and superior conjunctival
epithelium (3.55; P = 0.12). The MPI of fibrovascular
tissue from recurrent pterygium (73.75) was significantly higher than
the MPI of fibrovascular tissue from primary pterygium (7.3; P = 0.003).
conclusions. The finding of high levels of cellular proliferation in the
subepithelial fibrovascular layer of pterygium confirms that pterygium
is a disorder of excessive cellular proliferation and that the
fibrovascular layer is the site of cellular proliferation. Markedly
raised levels of cellular proliferation in recurrent pterygium tissue
suggest a clinical correlation between fibrovascular tissue
upregulation and pterygium recurrence after
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