Purchase this article with an account.
Qingwen Xu, Tamim Qaum, Anthony P. Adamis; Sensitive Blood–Retinal Barrier Breakdown Quantitation Using Evans Blue. Invest. Ophthalmol. Vis. Sci. 2001;42(3):789-794.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
purpose. This study investigated whether a nonradioactive dye, Evans blue, can be
adapted as a safe alternative to the isotope-dilution method for
quantitating blood–retinal barrier breakdown.
methods. Blood–retinal barrier breakdown was induced in rats with vascular
endothelial growth factor (VEGF) or through the induction of diabetes.
After allowing Evans blue to circulate in the vasculature, the dye was
cleared from the bloodstream with saline, citrate, or citrate-buffered
paraformaldehyde, and the efficacies of the perfusion solutions were
compared. Extravasated dye was detected at 620 nm and was normalized
against the time-averaged Evans blue plasma concentration, the
circulation time, and also against wet and dry retina weights.
results. Evans blue leakage from retinas treated with VEGF was 4.0-fold higher
than that of contralateral untreated eyes (n = 6 rats, P < 0.05). Retinal Evans blue leakage of eyes from
1-week diabetic animals (n = 11 retinas) was 1.7-fold
higher (P < 0.05) than that of nondiabetic controls
(n = 10 retinas). Intra-animal, inter-retina weights
showed significantly less variability (P < 0.05) with
the use of dry weights (11.2%, n = 74 retina pairs)
than with wet weights (20.5%, n = 93 retina pairs).
conclusions. The Evans blue dye technique can be modified to be as sensitive and
quantitative as the isotope-dilution method for measuring
blood–retinal barrier breakdown. The advantages of the Evans blue
technique are its safety, relative simplicity, and
This PDF is available to Subscribers Only