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Kristine S. Magabo, Joseph Horwitz, Joram Piatigorsky, Marc Kantorow; Expression of βB2-Crystallin mRNA and Protein in Retina, Brain, and Testis. Invest. Ophthalmol. Vis. Sci. 2000;41(10):3056-3060.
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purpose. To evaluate the expression of βB2-crystallin mRNA and
protein in rat, bovine, and human nonlens and nonocular tissues.
methods. βB2-crystallin mRNA levels were detected by RT-PCR.β
B2-crystallin protein was purified from rat and bovine
tissues by FPLC chromatography. FPLC fractions were analyzed by
immunoblotting. The identity of βB2-crystallin protein,
isolated from the retina, was confirmed by protein microsequencing.
results. βB2-crystallin transcript was detected in rat brain, rat
testis, and human retina by RT-PCR. βB2-crystallin
transcript was not found in rat lung, heart, ovary, spleen, thymus,
kidney, and liver or in human brain and testis.β
B2-crystallin protein was partially purified from and
its identity confirmed in rat brain, rat testis, and bovine retina. The
bovine retinal protein was further confirmed to be authenticβ
B2-crystallin by protein microsequencing.
conclusions. These results establish that βB2-crystallin mRNA and
protein are expressed in tissues outside of the lens and outside of the
eye including retina, brain, and testis. Extralenticular and
extraocular expression of βB2-crystallin, coupled with
its participation in phosphorylation pathways, suggests that it has
nonrefractive functions in these tissues.
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