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Ruth E. Swiderski, Jean L. Ross, John H. Fingert, Abbot F. Clark, Wallace L. M. Alward, Edwin M. Stone, Val C. Sheffield; Localization of MYOC Transcripts in Human Eye and Optic Nerve by In Situ Hybridization. Invest. Ophthalmol. Vis. Sci. 2000;41(11):3420-3428.
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purpose. To evaluate MYOC (myocilin) gene expression at the RNA
level in normal intact human eyes and optic nerve using in situ
methods. Normal human eyes and optic nerves from donors 62 to 83 years of age
with no history of glaucoma were fixed, embedded in paraffin, and
sectioned. Sections were hybridized with 35S-labeled sense
and antisense riboprobes derived from a full-length MYOC cDNA.
results. High levels of MYOC expression were observed throughout
the trabecular meshwork as well as in the most anterior nonfiltering
meshwork (Schwalbe’s line), in the scleral spur, and in the
endothelial lining of Schlemm’s canal. MYOC transcripts
were also detected in the anterior corneal stroma, in the ciliary
muscle, beneath the anterior border of the iris, in the iris stroma,
and in the sclera. Expression in the retrolaminar region of the optic
nerve was present in the pial septa that divide the nerve fiber
bundles, in the perivascular connective tissue surrounding the central
retinal vessels, and in the dura mater, arachnoid, and pia mater of the
meningeal sheath surrounding the optic nerve.
conclusions. MYOC gene expression in the trabecular meshwork,
Schlemm’s canal, scleral spur, and ciliary muscle indicates a
structural or functional role for myocilin in the regulation of aqueous
humor outflow that may influence intraocular pressure. MYOC expression in the optic nerve suggests that changes
in the structural, metabolic, or neurotropic support of the optic nerve may influence its susceptibility to glaucomatous
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