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Hiroshi Ohguro, Kei–ichi Ogawa, Tadao Maeda, Akiko Maeda, Ikuyo Maruyama; Cancer-Associated Retinopathy Induced by Both Anti-Recoverin and Anti-hsc70 Antibodies In Vivo. Invest. Ophthalmol. Vis. Sci. 1999;40(13):3160-3167.
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purpose. In a previous study, both recoverin and heat shock cognate protein 70
(hsc 70) were found as autoantigens recognized by sera from four
patients with cancer-associated retinopathy (CAR). This
observation suggested that autoimmune reactions against recoverin and
hsc 70 might be involved together in the pathogenesis of CAR. The
purpose of the present study is to investigate the effects of these
autoantibodies on retinas in vivo.
methods. Functional and morphologic properties of the retinas were evaluated
after anti-recoverin and/or anti-hsc 70 antibodies were intravitreously
injected into Lewis rats’ eyes.
results. Responses in electroretinogram (ERG) of eyes penetrated with anti-hsc
70 antibody were comparable with the control, but those with
anti-recoverin antibody were remarkably reduced during the 3-week
period after the injection. Such anti-recoverin antibody–induced
reduction was significantly enhanced by copenetration with anti-hsc 70
antibody. Immunofluorescence microscopy demonstrated that after
intravitreal injection, anti-recoverin antibody penetrated toward the
outer nuclear layer (ONL) and outer segments within 12 to 24 hours, and
the presence of the antibody in the retina diminished during the next
few days. Histopathology revealed significant thinning of the ONL and
inner nuclear layer (INL) in the affected retina in comparison with the
control. Throughout the ONL and INL, apoptotic cells were recognized by
TdT-dUTP terminal nick-end labeling. The antibody-induced retinal
dysfunction was effectively treated by administrations of either
corticosteroid or cyclosporin A.
conclusions. These observations suggest that anti-recoverin– and anti-hsc 70
antibody–induced retinal dysfunction in Lewis rat is a good model to
study the pathophysiology of CAR.
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