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Marcelo N. Ayala, Ralph Michael, Per G. Söderberg; Influence of Exposure Time for UV Radiation–Induced Cataract. Invest. Ophthalmol. Vis. Sci. 2000;41(11):3539-3543.
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purpose. It is believed that for a certain ultraviolet radiation (UVR) exposure,
the biologic effect depends on the product of irradiance and exposure
time (the reciprocity Bunsen–Roscoe law). The purpose of this study
was to investigate the validity of the reciprocity law for UVR-induced
methods. Two experiments were conducted. In the first one, 100 Sprague–Dawley
rats were exposed to UVR divided into five groups according to exposure
time: 7.5, 15, 30, 60, and 120 minutes. In the second experiment, 80
Sprague–Dawley rats were exposed to UVR divided into four groups
according to exposure time: 5, 7.5, 11, and 15 minutes. All the animals
were unilaterally exposed to the same dose of UVR (8 kJ/m2)
in the 300-nm wavelength region. One week after exposure both lenses
were removed to measure the intensity of forward light scattering and
for microphotography. Groups were compared by evaluating the difference
between exposed and nonexposed eyes.
results. The group exposed to UVR for 5 minutes had the lowest intensity of
forward light scattering. The highest intensity of forward light
scattering was found in the group that was exposed for 15 minutes. With
longer exposure intervals, the intensity of forward light scattering
decreased as the exposure time increased. No difference in intensity of
forward light scattering was found between the groups exposed for 60
and 120 minutes.
conclusions. Exposure time strongly influenced cataract formation after low-dose
UVR. In this model of UVR-induced cataract, the photochemical
reciprocity law was modulated by a biologic
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