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Linda D. Hazlett, Sharon A. McClellan, Xiaowen L. Rudner, Ronald P. Barrett; The Role of Langerhans Cells in Pseudomonas aeruginosa Infection. Invest. Ophthalmol. Vis. Sci. 2002;43(1):189-197. doi: https://doi.org/.
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purpose. Previous experimental studies have shown that extended-wear contact
lens usage results in a centripetal migration of Langerhans cells from
the conjunctiva into the central cornea. To test the consequences of
this, Langerhans cells were induced into the cornea before Pseudomonas aeruginosa infection in BALB/c mice that are
normally resistant (the cornea heals) and in C57BL/6 mice that are
susceptible (the cornea perforates) to bacterial challenge.
methods. Mean clinical scores, slit lamp examination, adenosine diphosphatase
(ADPase), and acid phosphatase staining as well as immunostaining with
DEC-205, B7-1, CD4, and interleukin-2 receptor (IL-2R) antibodies and
histopathologic, RT-PCR, and delayed-type hypersensitivity (DTH)
analyses were used to examine the effects on bacterial disease after
polystyrene bead induction of Langerhans cells into the cornea before
results. No difference in disease response was observed in bead- versus
sham-treated C57BL/6 mice after bacterial infection; however,
significant differences leading to corneal perforation were seen in
BALB/c mice that included an increased number of Langerhans cells in
the central cornea at 1 and 6 days after infection, an increased number
of B7-1+ (mature) Langerhans cells at 6 days after
infection, CD4+ and IL-2R+ T cells at 5 days
after infection, enhanced DTH, and increased mRNA levels for IFN-γ in
cornea and cervical lymph nodes. Alternately, levels of IL-4 were
significantly higher in the cornea and cervical lymph nodes of sham-
versus bead-treated animals.
conclusions. These data provide evidence that Langerhans cells are critical in the
innate immune response to P. aeruginosa and provide new
information regarding the mechanisms governing resistance versus
susceptibility to bacterial infection with this opportunistic
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