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Rachelle Merriman–Smith, Paul Donaldson, Joerg Kistler; Differential Expression of Facilitative Glucose Transporters GLUT1 and GLUT3 in the Lens. Invest. Ophthalmol. Vis. Sci. 1999;40(13):3224-3230.
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purpose. To determine the expression patterns for members of the facilitative
glucose transporter family (GLUT1–4) in the rat lens.
methods. An initial molecular profiling of GLUT expression in lens fiber cells
was achieved using reverse transcription–polymerase chain reaction
(RT-PCR). The presence of isoform-specific transcript detected by
RT-PCR was then confirmed using northern blot analysis and in situ
hybridization. The presence of transporter protein was verified by
western blot analysis and immunocytochemistry.
results. Transcripts for GLUT1 and GLUT3, but not for GLUT2 and GLUT4, were
detected by RT-PCR of fiber cell mRNA. Transcript for GLUT3, but not
for GLUT1, was detected by northern blot analysis of fiber cell total
RNA, indicating that GLUT3 was the predominant isoform in the fiber
cells. In situ hybridization and immunolocalization in rat lens
sections confirmed this result at the transcript and protein levels,
respectively. In contrast, GLUT1 was predominantly expressed in the
lens epithelium and only to a limited extent in the equatorial fiber
conclusions. GLUT1 and GLUT3 are differentially expressed in the rat lens. The
presence of the high-affinity transporter GLUT3 in fiber cells
indicates that these cells have the capacity to take up glucose
independently from the epithelium.
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