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Jun Yamada, Bruce R. Ksander, J. Wayne Streilein; Cytotoxic T Cells Play No Essential Role in Acute Rejection of Orthotopic Corneal Allografts in Mice. Invest. Ophthalmol. Vis. Sci. 2001;42(2):386-392.
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purpose. To determine whether cytotoxic T cells of the direct alloreactive type are
activated and responsible for early, acute failure of orthotopic
corneal allografts observed in eyes of C57BL/6 but not of BALB/c mice.
methods. Corneas from BALB/c and BALB.B mice were placed orthotopically in eyes
of C57BL/6 and β-2 microglobulin knockout mice (deficient in
CD8+ cytotoxic T cells). Graft fates were assessed
clinically, and the T lymphocytes of recipients were assayed for the
capacity to lyse target cells bearing donor major (MHC) and/or minor
histocompatibility (minor H) antigens (direct and indirect pathways,
results. Similar to BALB/c recipients, C57BL/6 mice with rejected cornea
allografts acquired donor minor H–specific T cells. Unlike BALB/c
recipients, C57BL/6 mice—both rejectors and acceptors—acquired donor
MHC-specific T cells. β-2 Microglobulin knockout mice showed
rejection of corneal allografts in a manner indistinguishable from
C57BL/6 mice, including early, acute rejection, yet T cells from β-2
microglobulin knockout recipients of corneal allografts displayed no
cytotoxic T cells specific for either donor MHC or minor H
conclusions. Although C57BL/6 mice acquired donor MHC-specific cytotoxic T cells
(direct alloreactive cells), neither these cells nor donor minor
H–specific cytotoxic T cells (indirect alloreactive cells) play any
essential role in corneal allograft rejection, including the early
acute failure uniquely observed in C57BL/6 eyes.
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