Purchase this article with an account.
Toshiyuki Nagamoto, Goro Eguchi, David C. Beebe; Alpha-Smooth Muscle Actin Expression in Cultured Lens Epithelial Cells. Invest. Ophthalmol. Vis. Sci. 2000;41(5):1122-1129.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
purpose. Lens epithelial cells transdifferentiate to myofibroblasts during the
formation of anterior subcapsular cataracts and secondary cataracts.
One of the defining characteristics of myofibroblasts is the expression
of α-smooth muscle actin (α-SMA). This study investigated some of
the factors that influence α-SMA expression in lens epithelial cells.
methods. Bovine, rabbit, and human lens epithelial explants or cells were
cultured with or without serum. Immunohistochemistry and immunoblotting
were used to detect and quantitate α-SMA expression.
results. Cells from all species studied expressed α-SMA in primary explant
culture with or without serum. Immunostaining for α-SMA first
appeared in a diffuse granular pattern, then accumulated at the cell
cortex, and eventually was detected along stress fibers. When lens
epithelial cells migrated onto cell-free regions of the capsule or were
transferred to a plastic culture dish, α-SMA expression increased
significantly. Expression of α-SMA positively correlated with cell
size and cell migration.
conclusions. Expression of α-SMA is a common feature of cultured mammalian lens
epithelial cells. Because α-SMA expression occurred without the
addition of exogenous factors, the fibrosis seen in anterior
subcapsular cataracts or secondary cataracts may reflect the intrinsic
properties of lens epithelial cells. Interaction between lens
epithelial cells and their substratum appears to be an important
regulator of myofibroblast formation. Understanding the factors that
regulate α-SMA expression in lens epithelial cells could lead to the
development of methods for preventing secondary cataracts and anterior
This PDF is available to Subscribers Only