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Partha Mukhopadhyay, Lijun Bian, Hulian Yin, Parimal Bhattacherjee, Christopher A. Paterson; Localization of EP1 and FP Receptors in Human Ocular Tissues by In Situ Hybridization. Invest. Ophthalmol. Vis. Sci. 2001;42(2):424-428.
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purpose. To examine the expression and localization of EP1 and FP
receptor mRNAs in normal human ocular tissues by in situ hybridization.
methods. Digoxigenin-labeled human EP1 and FP receptor antisense and
sense riboprobes were used for in situ hybridization on paraffin
sections of normal human eye tissue.
results. In situ hybridization revealed the presence of high levels of both
EP1 and FP receptor mRNA transcripts in the blood vessels
of iris, ciliary body, and choroid. Both the endothelial and smooth
muscle cells of blood vessels demonstrated intense hybridization
signals corresponding to EP1 receptor mRNA transcript.
EP1 receptor hybridization signals were present in all the
muscle fibers of the ciliary body. In the retina, hybridization signals
for EP1 receptors were observed in photoreceptors and both
nuclear layers and in ganglion cells. The hybridization signals
corresponding to FP receptor transcript were similar to those of
EP1 receptors in the iris tissues. In the ciliary muscle,
FP receptor mRNA transcript was predominantly present in the circular
muscle and in the collagenous connective tissues; no hybridization
signal for this receptor was observed in the retina.
conclusions. The wide distribution of EP1 and FP receptor mRNAs in human
ocular tissues appears to be localized in the functional sites of the
respective receptor agonists. Selective localization of FP receptor
mRNA in the circular muscles and collagenous connective tissues of the
ciliary body suggests their involvement in the increased uveoscleral
outflow of aqueous humor by PGF2α.
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