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Ippei Takasu, Fumio Shiraga, Toshio Okanouchi, Yozo Tsuchida, Hiroshi Ohtsuki; Evaluation of Leukocyte Dynamics in Choroidal Circulation with Indocyanine Green–Stained Leukocytes. Invest. Ophthalmol. Vis. Sci. 2000;41(10):2844-2848.
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purpose. To develop a new method with which to visualize leukocytes moving
through the choroidal vessels of pigmented animals and enable the
evaluation of leukocyte dynamics in the choroidal microcirculation.
methods. Pigmented rabbits and monkeys were used in this study. Leukocytes,
collected by centrifugal separation of autologous blood, were stained
with indocyanine green (ICG) dye. The ICG-stained leukocyte fluid was
injected into the vein, and the fundus image was obtained with a
scanning laser ophthalmoscope. The image was recorded on videotapes and
analyzed with a personal computer-based image analysis system.
results. In pigmented rabbits, fluorescent leukocytes moving in the choroidal
circulation were clearly visible for more than 1 hour. In monkeys,
distinct fluorescent dots were seen moving approximately 50 to 200 μm
in the foveal avascular zone for more than 30 minutes after the
injection of the ICG-stained leukocyte fluid. Dim fluorescent dots were
seen moving in the fundus. Although the movement of these dim dots was
difficult to trace, they seemed to be moving in the choroidal vessels.
In the rabbits, the mean flow velocity of leukocytes moving without
plugging was 0.48 ± 0.14 mm/sec in the peripheral
choriocapillaris. In the monkeys, the mean flow velocity of distinct
fluorescent leukocytes without plugging was 2.45 ± 0.48 mm/sec in
the posterior choroid.
conclusions. In pigmented rabbits and monkeys, this method allows visualization of
leukocytes passing through the choroidal vessels and provides a new way
to investigate, noninvasively and in vivo, leukocyte dynamics in the
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