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Makoto Nakamura, Dhirendra P. Singh, Eri Kubo, Leo T. Chylack, Toshimichi Shinohara; LEDGF: Survival of Embryonic Chick Retinal Photoreceptor Cells. Invest. Ophthalmol. Vis. Sci. 2000;41(5):1168-1175.
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purpose. Lens epithelium–derived growth factor (LEDGF) is a novel adhesive,
survival, and growth factor for lens epithelial cells, keratinocytes,
fibroblasts, and cos7 cells. In the presence of LEDGF, these cells
acquire resistance to environmental stresses, and in the absence of
LEDGF they die. The effects of LEDGF on survival of embryonic
chick retinal photoreceptor cells under serum starvation and heat
stress were studied.
methods. The expression pattern of LEDGF in embryonic chick retinal
photoreceptor cells was investigated with protein blot analysis and
immunohistochemistry using antibodies (Abs) to LEDGF. Retinal cells
were cultured in serum-free medium for up to 6 days in the presence of
varying amounts of LEDGF at 37° or 41°C. The photoreceptor cells
were immunostained with Abs to arrestin and counted to evaluate the
photoreceptor cell viability. Heat shock proteins in the cultured cells
were quantified by protein blot analysis with Ab probes and
semiquantitative reverse transcription–polymerase chain reaction
results. LEDGF was found predominantly in the nucleus of neuroretinal cells,
including photoreceptor cells. In the presence of LEDGF, photoreceptor
cells manifested increased resistance to serum starvation and thermal
stress and survived for a longer period. The levels of heat shock
protein 90 were elevated in those cells. Most retinal cells died in the
absence of LEDGF.
conclusions. LEDGF enhanced survival of retinal photoreceptor cells under serum
starvation and heat stress. Thus, LEDGF has a potency to enhance
survival of neuronal cell types against environmental stresses, and it
may be applicable as a therapeutic agent for those
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