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Hao Wang, Debra S. Leonard, Alessandro A. Castellarin, Itsuro Tsukahara, Yoshihiko Ninomiya, Fumihiko Yagi, Noounanong Cheewatrakoolpong, Ilene K. Sugino, Marco A. Zarbin; Short-Term Study of Allogeneic Retinal Pigment Epithelium Transplants onto Debrided Bruch’s Membrane. Invest. Ophthalmol. Vis. Sci. 2001;42(12):2990-2999.
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purpose. To investigate the survival and behavior of retinal pigment epithelium
(RPE) microaggregates transplanted onto hydraulically debrided Bruch’s
membrane and to compare results of using three different vehicles for
methods. RPE microaggregates obtained from male cats were transplanted onto the
tapetal area of female cats after native RPE was debrided. For the
control, one of three vehicles was introduced into the debridements.
Each transplant or control specimen was analyzed histologically and
immunohistochemically. Transplanted male RPE cells were identified by
in situ labeling of the cat Y chromosome.
results. Histologically, significant numbers of condensed, darkly stained RPE
nuclei were observed in all transplants compared with few
TUNEL-positive RPE cells. Cellular retinaldehyde-binding protein was
present up to day 7 in all RPE cells in transplants. In both transplant
and control specimens, the antibody against the Ki-67 nuclear antigen
labeled some RPE cells at day 3. TUNEL-positive outer nuclear layer
nuclei were most frequently observed at day 1, but were much less
frequent at 7 days in both transplant and control specimens.
conclusions. Transplanted RPE appeared to retain at least some markers of
differentiation up to 7 days after surgery. Some proliferation of
transplanted RPE cells was also seen. Apoptotic cell death of
transplanted RPE, as judged by TUNEL staining was observed rarely. RPE
transplants imposed no adverse effect on the overlying retina. RPE
survival appeared to be similar with each of the three vehicles for
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