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Christopher L. Cubitt, Robert N. Lausch, John E. Oakes; Synthesis of Type II Interleukin-1 Receptors by Human Corneal Epithelial Cells but not by Keratocytes. Invest. Ophthalmol. Vis. Sci. 2001;42(3):701-704. doi: https://doi.org/.
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purpose. The purpose of this study was to determine whether human corneal
epithelial cells and keratocytes synthesize both the soluble and
membrane forms of the type II IL-1 receptor (IL-1RII).
methods. Primary cell cultures of human corneal epithelial cells and keratocytes
were established from human corneas. RT-PCR was used to analyze cell
cultures for expression of IL-1RII mRNA. The capacity of corneal cells
to synthesize membrane-bound IL-1RII was determined by
immunofluorescence microscopy, whereas ELISA was used to quantitate
synthesis of soluble IL-1RII after IL-1α and TNF-α stimulation.
results. Corneal epithelial cells expressed IL-1RII mRNA. The cells also stained
positive for membrane-bound IL-1RII, and media harvested from
epithelial cell cultures contained up to 50 pg/ml of soluble IL-1RII.
Both IL-1α and TNF-α significantly enhanced the amounts of soluble
IL-1RII released from epithelial cell surfaces. In contrast to
epithelial cells, corneal keratocytes did not express IL-1RII mRNA.
Membrane-bound IL-1RII was not detected on keratocytes, nor was soluble
IL-1RII detected in culture media harvested from these cells.
conclusions. Human corneal epithelial cells but not corneal keratocytes synthesize
both membrane and soluble forms of IL-1RII. Because both forms of
IL-1RII can function as IL-1α antagonists, the results suggest that
human corneal epithelial cells but not corneal keratocytes have evolved
the capacity to dampen IL-1α responses through the production of
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