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Mathias W. Seeliger, Kristina Narfström; Functional Assessment of the Regional Distribution of Disease in a Cat Model of Hereditary Retinal Degeneration. Invest. Ophthalmol. Vis. Sci. 2000;41(7):1998-2005.
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purpose. To establish a method for the recording of multifocal
electroretinograms (MF–ERGs) in animals under fundus control using a
scanning-laser ophthalmoscope (SLO) and to analyze the spatial
distribution of disease in a strain of Abyssinian cats with a
recessively inherited rod-cone degeneration (ARCD).
methods. Four normal and 12 Abyssinian cats at four different clinical stages of
ARCD were examined with the RETIscan MF–ERG system using 61 hexagonal
elements within a visual field of approximately 30° radius. The
stimulus pattern was generated by the green laser beam (515 nm) of a
Heidelberg Engineering HRA SLO, whose power was reduced with a
Schott long-pass filter allowing for simultaneous infrared fundus
results. Topographical recordings could be obtained in all animals except one in
stage 4. Amplitudes were minimal at the optic disc and had a slight
maximum at the area centralis. Implicit times had a tendency to lower
values in the central region, most pronounced in progressed stages of
ARCD. The clinical stages of ARCD correlated with a successive
generalized loss of amplitude and a rise in implicit time. Without a
decrease in retinal illuminance, topographical landmarks like the optic
disc were no longer detectable, pointing to stray light as a possible
conclusions. It was demonstrated that topographical MF–ERG recordings can be
obtained in an animal model under fundus control using SLO stimulation.
The appearance of retinal landmarks was found to be dependent on
sufficient attenuation of laser power. Because the changes in ARCD are
more patchy than in human retinitis pigmentosa (RP), a generalized loss
of function was detected. However, like in RP, the central area was
found to retain a better function than the periphery, especially in
later stages of the disease. In summary, fundus controlled methods like
the one presented will greatly improve the reliability of MF–ERG
in future research on glaucoma, transplantation studies, and evaluation
of gene therapy.
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