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Mei–Lan Ko, Dan–Ning Hu, Robert Ritch, Sansar C. Sharma; The Combined Effect of Brain-Derived Neurotrophic Factor and a Free Radical Scavenger in Experimental Glaucoma. Invest. Ophthalmol. Vis. Sci. 2000;41(10):2967-2971.
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purpose. Brain-derived neurotrophic factor (BDNF) had a limited effect on the
survival of retinal ganglion cells (RGCs) in rats’ eyes with elevated
intraocular pressure (IOP). The combined treatment of BDNF and
a nonspecific free radical scavenger N-tert-butyl-(2-sulfophenyal)-nitrone (S-PBN) was
investigated on the RGCs in hypertensive eyes of rats.
methods. Adult Wistar rats were separated into five groups: BDNF (0.5 μg) +
S-PBN; BDNF (1.0 μg) + S-PBN; BDNF (1.0 μg); S-PBN; and
phosphate-buffered saline. Right eyes served as normal
controls (n = 10). RGCs were labeled with 5%
Fluoro Gold; injected into the superior colliculus. Three days
after intratectal injection, the episcleral veins of the left eyes were
cauterized. Intravitreal injection of BDNF was performed on days 5, 13,
21, and 29 after IOP elevation. S-PBN was injected intraperitoneally
(100 mg/kg body wt) every 12 hours starting 30 minutes after
results. The survival of RGCs using BDNF treatment alone in moderately
hypertensive eyes and systemic administration of S-PBN alone did not
significantly rescue the RGCs. However, the combination of BDNF and
S-PBN increased the survival of RGCs to 90.1%.
conclusions. Trophic factors and antioxidants have synergistic effects on rescuing
RGCs from death in eyes with elevated IOP. Further studies of different
combined treatment therapies may provide avenues to save RGCs from
death in eyes with elevated IOP.
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