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Hongqing Q. Ye, Masaharu Maeda, Fu–Shin X. Yu, Dimitri T. Azar; Differential Expression of MT1-MMP (MMP-14) and Collagenase III (MMP-13) Genes in Normal and Wounded Rat Corneas. Invest. Ophthalmol. Vis. Sci. 2000;41(10):2894-2899.
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purpose. Several members of the matrix metalloproteinase (MMP) group have been
identified in the rat cornea during corneal wound healing. The aim of
the present study was to identify additional members of the MMP gene
family in the rat cornea and localize the expression of membrane type-1
matrix metalloproteinase (MT1-MMP; MMP-14) and collagenase III (MMP-13)
in normal and wounded corneas.
methods. Adult rats underwent laser keratectomy on the right eye. Unwounded left
eyes were normal controls. Corneas were collected and processed at
different times post-wounding. Reverse transcription–polymerase chain
reaction (RT-PCR) and DNA sequencing were used to discover the MMP
genes expressed in the corneas. In situ hybridization was performed to
localize the mRNA expression of MMP-14 and MMP-13.
results. MMP-13 mRNA was detected in epithelial cells of wounded corneas, but
not in normal controls; MMP-14 was found in both normal and wounded
corneas. MMP-14 mRNA was expressed predominantly in the stromal
keratocytes and rarely in the basal epithelial cells in normal and
wounded corneas. MMP-13 mRNA was localized exclusively to basal cells
of the epithelium at the wounded area from 6 hours to 3 days after
conclusions. MMP-14 and MMP-13 expression in rat corneas parallels that of
gelatinases A and B, respectively. MMP-13 may play an important role in
the gelatinase B–associated proteolytic cascade that allows rapid
turnover of the extracellular matrix (ECM) components during corneal
wound healing. MMP-14 may contribute to removing abnormal ECM
components through activation of gelatinase A in rat
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