Purchase this article with an account.
Jody A. Rada, Debora L. Nickla, David Troilo; Decreased Proteoglycan Synthesis Associated with Form Deprivation Myopia in Mature Primate Eyes. Invest. Ophthalmol. Vis. Sci. 2000;41(8):2050-2058.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
purpose. The rate of proteoglycan synthesis was measured in the scleras of
adolescent marmosets that had undergone monocular form deprivation to
characterize the scleral extracellular matrix changes associated with
the development of myopia in a mature primate.
methods. Form deprivation myopia was induced in adolescent marmosets by
unilateral lid suture for an average of 108 days. After the lids were
reopened, the axial lengths and refractions were measured at intervals
for up to 39 weeks. At the end of the study period, sclera were
isolated and immediately radiolabeled with 35SO4 in organ culture. Proteoglycan synthesis
rates were determined by measurement of 35SO4 incorporation into cetylpyridinium chloride–precipitable
glycosaminoglycans after digestion of the scleral samples with
proteinase K. Collagen content was determined by measurement of total
hydroxyproline in scleral digests. Newly synthesized proteoglycans were
separated on a Sepharose CL-4B molecular sieve column and identified by
their core proteins by Western blot analyses.
results. Lid suture resulted in myopia due to a significant increase in vitreous
chamber depth. After Sepharose CL-4B chromatography, newly synthesized
scleral proteoglycans isolated from normal, form-deprived, and
contralateral control eyes, resolved into one major peak that eluted in
the position of decorin, a small chondroitin-dermatan sulfate
proteoglycan. After digestion of the major peak with chondroitinase
ABC, an approximately 45-kDa core protein was detected by Western blot
analyses, confirming the presence of decorin. Form deprivation resulted
in a significant reduction in the rate of proteoglycan synthesis in the
posterior sclera (−43.55%, P ≤ 0.001).
Proteoglycan synthesis was also significantly reduced in the posterior
sclera of form-deprived eyes relative to total collagen content
(−36.19%, P ≤ 0.01) and was negatively
correlated with the rate of vitreous chamber elongation in the deprived
eye (r 2 = 0.779, P ≤ 0.05).
conclusions. Significant extracellular matrix remodeling occurs in the posterior
sclera of the adolescent primate eye during vitreous chamber elongation
and myopia development. The negative correlation between vitreous
chamber elongation rates and the synthesis rates of decorin in
form-deprived eyes suggests that proteoglycan synthesis within the
posterior sclera plays a role in the regulation of ocular size and
refraction in the adolescent marmoset.
This PDF is available to Subscribers Only