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Si–Wei You, Kwok–Fai So, Henry K. Yip; Axonal Regeneration of Retinal Ganglion Cells Depending on the Distance of Axotomy in Adult Hamsters. Invest. Ophthalmol. Vis. Sci. 2000;41(10):3165-3170.
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purpose. To examine the relationship between the distance of axotomy and axonal
regeneration of injured retinal ganglion cells (RGCs) systematically
and the effect of a predegenerated (pretransected or precrushed)
peripheral nerve (PN) graft on axonal regeneration of RGCs axotomized
at a definite distance (0.5 mm from the optic disc) in comparison with
a normal PN graft.
methods. The optic nerve (ON) was transected intraorbitally at 0.5, 1, 1.5, 2,
or 3 mm or intracranially at 6 to 8 mm from the optic disc, and a PN
graft was transplanted onto the ocular ON stump in adult hamsters. Four
weeks after grafting, the number of RGCs regenerating their injured
axons into the PN graft was investigated in all animals.
results. The number of regenerating RGCs decreased significantly when the
distance of axotomy increased from 0.5 to 7 mm. A precrushed PN graft
was shown to enhance more injured RGCs to regenerate axons than a
normal or pretransected PN graft.
conclusions. The distance of axotomy on the ON of adult hamsters is critical in
determining the number of regenerating RGCs. Thus, experimental
strategies to repair the damaged ON by PN transplantation is to attach
a precrushed PN graft as close to the optic disc as possible to obtain
optimal axonal regeneration of the axotomized
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