(
A) Absorbance of native (upper curve:λ
max, 282 nm) and mock chicken aqueous humor
(λ
max, 269 nm; i.e., phosphate buffer
containing protein, tyrosine, tryptophan, and ascorbic acid according
to
Table 1 ). Note the marked difference in UV absorbance between the
curves. (
B) The upper curve (λ
max,
288 nm) i.e., the mock aqueous absorbance subtracted from the native
one in
Figure 1 , imitates the absorbance of uric acid shown in
the lower curve (λ
max, 290 nm; pH 7.3) at an
arbitrarily chosen concentration of 100 μmol/l. (
C) UV
absorbance of one native chicken aqueous collection (
upper
curve). Mock aqueous containing protein, tyrosine, tryptophan, and
ascorbate according to mean chicken values in
Table 1 (
lower
curve) was supplemented with 195 μmol/l uric acid (
middle
curve), which was the content of this particular native sample.
The curves showed λ
max at 288, 269, and 288 nm,
respectively. (
D) UV absorbance of one native turkey aqueous
collection (
upper curve). Mock aqueous containing protein,
tyrosine, tryptophan, and ascorbate according to mean turkey values in
Table 1 (
lower curve) was supplemented with 167 μmol/l
uric acid (
middle curve), which was the content of this
particular native sample. The curves showedλ
max at 281, 271, and 287 nm, respectively.