Purchase this article with an account.
Kunihiko Nakamura, Daijiro Kurosaka, Mami Yoshino, Takeshi Oshima, Hiroyo Kurosaka; Injured Corneal Epithelial Cells Promote Myodifferentiation of Corneal Fibroblasts. Invest. Ophthalmol. Vis. Sci. 2002;43(8):2603-2608.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
purpose. To determine whether injured corneal epithelial cells stimulate myodifferentiation in corneal fibroblasts and whether transforming growth factor (TGF)-β is involved.
methods. Rabbit corneal fibroblasts were cultured on collagen gel, with or without cocultured corneal epithelial cells or with partially scraped epithelial cells, on a companion plate separated by a permeable membrane. To evaluate fibroblast-induced gel contraction, gel thickness was measured daily relative to the original thickness. Total fibroblasts on the gel were counted. Myofibroblasts were counted by using immunocytochemical identification with anti-α-smooth muscle actin (α-SMA). TGF-β was assayed in the media on days 3 and 6. These procedures also were performed in the presence of anti-TGF-β antibody.
results. Gel contraction, α-SMA-positive cells, and total cell number were significantly greater on gels with injured epithelial cells than on gels without epithelial cells or with uninjured epithelial cells, as was TGF-β concentration in the media. Anti-TGF-β antibody eliminated these differences.
conclusions. Injured epithelial cells stimulate myodifferentiation in fibroblasts through one or more soluble factors, including TGF-β.
This PDF is available to Subscribers Only