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Chi–Chun Lai, Peter Gouras, Kentaro Doi, Stephen H. Tsang, Stephen P. Goff, Paul Ashton; Local Immunosuppression Prolongs Survival of RPE Xenografts Labeled by Retroviral Gene Transfer. Invest. Ophthalmol. Vis. Sci. 2000;41(10):3134-3141.
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purpose. To determine whether local immunosuppression with Cyclosporin A can
influence the survival of human fetal retinal pigment epithelium (RPE)
xenografts in the rabbit’s subretinal space.
methods. Cultured human fetal RPE cells were transduced with the gene for green
fluorescent protein (GFP) using a lentiviral vector. The RPE was
transplanted into the subretinal space of rabbits that received
intravitreal cyclosporine either by weekly injections (0.25–0.5 mg) or
by slow release (approximately 2 μg/d) from a capsule sutured into
the vitreal cavity after prior cryopexy. The transplanted RPE was
followed by GFP fluorescence scanning laser ophthalmoscopy and by
histology of the transplant site.
results. RPE xenografts in eyes receiving intravitreal cyclosporine survived
longer (several months) than they did in control eyes without
cyclosporine. Survival was as long with slow release capsules as it was
with weekly intravitreal injections at much higher concentrations of
conclusions. Local immunosuppression of the eye with cyclosporine prolongs the
survival of RPE xenografts in the subretinal space of rabbits, implying
that rejection involves activated T lymphocytes. Local
immunosuppression with slow release capsules is as effective as weekly
injections at much higher concentrations.
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