Purchase this article with an account.
Arman Tehrani, Thomas H. Wheeler–Schilling, Elke Guenther; Coexpression Patterns of mGLuR mRNAs in Rat Retinal Ganglion Cells: A Single-Cell RT-PCR Study. Invest. Ophthalmol. Vis. Sci. 2000;41(1):314-319. doi: https://doi.org/.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
purpose. Eight different subunits of metabotropic glutamate receptors (mGluRs)
are known to date. mGluRs have been linked to an extensive list of
neuromodulatory effects, depending on which intracellular or
membrane-bound effector system is activated. Activation of mGluRs can
influence neuronal activity and can result in changes of intracellular
Ca2+ homeostasis—that is, changes in factors that are
known to be crucial for cellular differentiation and cell death.
Because mGluRs are known in modulating both intracellular and
intercellular activities, this study was designed to determine which
types of mGluRs are coexpressed in a neuron and whether distinct
coexpression patterns can be found that reflect the different
physiological requirements of a neuron at different stages of
development and to learn whether neuronal injury results in adaptive
changes of mGluR expression.
methods. Juvenile and adult rat retinal ganglion cells (RGCs) and adult RGCs
after axotomy were analyzed for their gene expression pattern of mGluRs
by single-cell reverse transcription–polymerase chain reaction
results. Adult RGCs predominantly expressed one or two different mGluR mRNAs,
whereas juvenile RGCs coexpressed two and more. mGluR3, -5, and -7
mRNAs were found more frequently in juvenile than in adult RGCs. mGluR6
was detected in juvenile RGCs in low abundance but never in adult RGCs.
However, mGluR6 was expressed in adult RGCs after axotomy. mGluR1 and
-7 were also found more frequently in axotomized RGCs than in the adult
conclusions. All types of mGluR mRNAs are expressed in RGCs. This is in contrast
with previous in situ hybridization and immunohistochemical studies in
which expression of mGluR3, -5, and -6 was not reported. The expression
of some mGluR mRNAs seems to be developmental, although no distinct
copatterns were found.
This PDF is available to Subscribers Only